ATG14/Barkor antibody 28021-1-AP from Proteintech Group Inc

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Cells
August 04 2022

Fig 1: LPS induces autophagy in BV2 cells. BV2 cells were treated with LPS (1 µg/mL) for 2, 4, 6, 12, and 24 h (A–F). (A,B) Protein levels of LC3 and SQSTM1/p62 were examined using immunoblotting. Representative immunoblots are presented in (A), and quantification results are presented as the means ± SEM in (B) (n = 4); (C,D) Immunofluorescence staining was conducted at 12 and 24 h after LPS treatment with anti-LC3 antibody (green) and DAPI (blue). Representative images are shown in (C) (Scale bar, 20 µm), and quantification of the dot– or ring–shaped LC3 signals (representing autophagosomes) are shown in (D) (n = 12); (E,F) ATG7, ATG14, ATG16, and Beclin-1 levels were examined using immunoblotting. Representative immunoblots are presented in (E), and quantification results are presented as the means ± SEM in (F) (n = 3 for ATG7 and ATG14, n = 4 for ATG16 and Beclin-1). (G,H) BV2 cells were pretreated with Bafilomycin A1 (Baf, 50 nM) or chloroquine (CQ, 5 µM) for 2 h and then treated with LPS (500 ng/mL) for 24 h, and level of LC3 was examined using immunoblotting; (I,J) BV2 cells were treated with LPS (100 ng/mL) for 12 h in the presence or absence of TLR4-IN-C34 (5 and 10 µM), and LC3 levels were examined using immunoblotting. Representative immunoblots are presented in (H) and (I), and quantification data are presented in (G) and (J) (n = 4). * p < 0.05, ** p < 0.01 vs. PBS-treated control cells, ## p < 0.01 between indicated groups using one–way ANOVA with Student–Newman–Keuls test.
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Heliyon
February 01 2023

Fig 2: (A) Protein expressions of GABRP and autophagic parameters in ICP placentas and normal control. (B) There were significantly higher protein expressions of GABRP in placentas with ICP compared with control group (P < 0.05), and there was significant difference between mild ICP group and severe ICP group (P < 0.05). No difference of LC3 expression was found in the comparison between ICP group and control group. There were significantly higher protein expressions of ATG 14 expression in placentas of ICP patients compared with control group (P < 0.05). (C) The expression of GABRP mRNA were significantly increased in ICP groups compared with control (P < 0.05), there was significant difference between mild ICP group and severe ICP group. In the comparison of LC3 and ATG14 mRNA expression, severe ICP group had increased expressions of these autophagic markers compared with control group. The uncropped Western blot picture are shown in Supplementary Fig. S1.
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Heliyon
February 01 2023

Fig 3: (A) (B) (C) Expressions of GABRP in placental tissue by immunochemistry in normal pregnancy, mild ICP patients and severe ICP patients. (D) (E) (F) Expressions of LC3 in placental tissue by immunochemistry in normal pregnancy, mild ICP patients and severe ICP patients. (G) (H) (I) Expressions of ATG14 in placental tissue by immunochemistry in normal pregnancy, mild ICP patients and severe ICP patients. (J) The AOD of GABRP was significantly increased in severe ICP group compared with control (P < 0.05), there was significant difference between mild ICP group and severe ICP group. In the comparison of AOD of LC3 and ATG14 expression, severe ICP group had increased expressions of these autophagic markers compared with control group.

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ATG14/Barkor antibody

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The ATG14/Barkor antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of ATG14/Barkor. This antibody recognizes recombinant protein antigen. The ATG14/Barkor antibody has been validated for the following applications: WB, IP, IHC, ELISA analysis

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Heliyon

ATG14/Barkor antibody from Proteintech Group Inc

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Proteintech Group Inc

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ATG14/Barkor antibody from Proteintech Group Inc

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